Abstract: In order to explore the static and dynamic characteristics of the cable-stayed bridge with large-tonnage spherical steel bearings, a load test was conducted on a long span cable-stayed bridge spanning railways that adopted large-tonnage spherical steel bearings. Through simulation analysis of the bridge structure, static and dynamic load tests, the static and dynamic characteristic changes of the bridge with large-tonnage spherical steel bearings and the bridge with traditional bearings under ideal conditions and test loads were compared, and the actual working condition of the bridge with large-tonnage spherical steel bearings was analyzed. The test results show that the actual load-bearing capacity of the bridge meets the design requirements, and the safety reserve is relatively reasonable. The deflection verification coefficients of each test section range from 0.71 to 0.84, the strain verification coefficients range from 0.62 to 0.78, and the verification coefficients of the cable force increment range from 0.91 to 0.92. The longitudinal horizontal displacement at the top of the main tower is basically linearly related to the loading level, with a verification coefficient of 0.70. The measured vibration modes are basically consistent with the theoretical vibration modes. The measured natural frequency of the bridge is higher than the theoretical calculated values. The measured damping ratio is within the normal range. The measured impact coefficient is smaller than the theoretical value. This indicates that the dynamic characteristics of the bridge comply with the structural design requirements, the structure is in a normal elastic working state, the actual stiffness is greater than the theoretical stiffness, and the structure has a good ability to dissipate external energy input, with a good driving capacity on the bridge deck. Compared with bridges of the same type, the calibration coefficient of the large-tonnage spherical steel bearing cable-stayed bridge is smaller. The reason for the smaller deflection calibration coefficient is that its own structural design has a relatively large excess bearing capacity. At the same time, the large-tonnage spherical steel bearing is set up, which has a stronger bearing capacity for the upper main beam structure and better overall stiffness. The dispersion of the strain calibration coefficient is relatively high because the theoretical model is in an ideal state. In on-site strain detection, the quality of concrete and other factors has a significant impact, resulting in a considerable deviation between the actual force and the theoretical calculation, as well as a relatively uneven stress distribution. In the dynamic load test, the measured vibration modes of the two cable-stayed bridges were both symmetrical in the first and third order vibration modes, and antisymmetric in the second order vibration mode, which remained consistent with the theoretical vibration mode.