Abstract: The breeding of speculation is still one of the difficult problems that block the green supply chain of characteristic agricultural products and threaten the quality and safety of agricultural products. Therefore, it is of great significance to further clarify the evolution mechanism and influencing factors of multi-subject behavior to reduce the green phenomenon of speculation in the supply chain so as to achieve the goal of green transformation in the supply chain of featured agricultural products. In this study, a three-party dynamic evolution game model with consumers, agriculture-related enterprises and farmers is constructed as the main body, the strategy selection and system stability of all parties are analyzed, and the influence of each element on the subject's decision is discussed. The results show that: the choice of consumers has an inhibitory effect on the speculative behavior of farmers; When farmers' defective product testing ability is weak, farmers' strategies tend to be more conservative, while with the improvement of testing ability, farmers' strategies gradually turn to be more active and cooperative;The improvement of the detection probability of defective products helps to ensure the quality of agricultural products and enhance the trust of consumers.