Abstract: Coal gangue, a solid waste generated during coal mining, is characterized by its loose texture, making it susceptible to wind erosion, which results in significant dust production and environmental pollution.The use of Paenibacillus mucilaginosus for efficient cementation and solidification treatment of coal gangue particles improves the resistance of coal gangue to wind erosion, thereby reducing environmental and atmospheric pollution.Theresponse surface methodology was employed to optimize the cultivation conditions for carbonic anhydrase production by Paenibacillus mucilaginosus.The optimal parameters were identified as a temperature of 31.2 ℃,a cultivation time of 48 hours, a liquid volume of 114.7 mL,and an inoculation amount of 14%,achieving an enzyme activity of 0.012 26 U.The experimental results show that temperature, liquid volume, cultivation time and inoculation amount have significant effects on enzyme activity, and there are significant interactions between temperature and liquid volume, and between inoculation amount and liquid volume.In simulated wind speed tests ranging from levels 4～6,the mass loss in the microbiologically treated group was markedly lower than that of the control group.Specifically, at a wind speed of 12 m/s, the mass loss of coal gangue samples treated with Paenibacillus mucilaginosus ranged only between 2.0～7.5 g.These findings indicate that the microbial consolidation layer significantly enhances the anti-wind erosion properties of coal gangue, demonstrating its potential for effectively suppressing wind erosion and dust generation in practical engineering applications.